Characterization of Yellow Pigmented Bacteria Associated with Gracilaria sp.

Arina Tri Lunggani, Susianna Purwantisari, Siti Nur Jannah


Research on the kinship analysis of endophytic bacterial  isolated from Gracillaria sp has been carried out. The presence of bacteria associated with Gracilaria sp. has enabled the use of these bacteria as a source of new bioactive compounds, such as biopigments. The research aims to isolated bacteria from Gracilaria sp., screened their symbiont bacteria that could potentially produce pigments. Sampling Gracilaria sp. conducted in the waters of the Island of  Karimunjawa, Jepara. Furthermore, bacterial isolation was carried out, screening for pigment-producing bacteria and 16S rRNA sequence analysis. Research result showed that the symbiont bacteria isolate TK 373 produced consistent pigments after several regenerations, in several types of growth media incubated at room temperature. The results of 16S rDNA identification showed that the TK 373 isolate had the closest relationship with  Pseudoalteromonas sp. with  98.72 % homology.

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G. M. Cragg, D. J. Newman, and K. M. Snader. 1997. Natural products in drug discovery and development. J.ournal of Natural Products, vol. 60, no. 1, pp. 52–60.

Smit, A.J., 2004. Medicinal and pharmaceutical uses of seaweed natural products: A review. Journal of Applied Phycology, 16: 245-262.

Ismail, A., Ktari, L., Ahmed, M., Bolhuis, H., Bouhaouala-Zahar, B., Stal, L.J., Boudabbous, A. and El Bour, M., 2018. Heterotrophic bacteria associated with the green alga Ulva rigida: identification and antimicrobial potential. Journal of Applied Phycology, pp.1-17.

E.M. Brown, P.J. Allsopp, P.J. Magee, C.I. Gill, S. Nitecki, C.R. Strain, E.M. McSorley, Seaweed and human health. 2014. Nutr. Rev. 72 (3) : 205–216

Peng, C., Hong-BO, S., Di, X., & Song, Q. 2009. Progress in Gracilaria biology and developmental utilization: main issues and prospective. Reviews in Fisheries Science, 17(4), 494-504.

Chakdar, H., & Pabbi, S. 2017. Algal pigments for human health and cosmeceuticals. In Algal green chemistry (pp. 171-188). Elsevier.

Yuan, S., Duan, Z., Lu, Y., Ma, X., & Wang, S. 2018. Optimization of decolorization process in agar production from Gracilaria lemaneiformis and evaluation of antioxidant activities of the extract rich in natural pigments. 3 Biotech, 8(1), 8.

Goecke F, Thiel V, Wiese J, Labes A, Imhoff JF . 2013. Algae as an important environment for bacteria—phylogenetic relationships among new bacterial species isolated from algae. Phycologia 52:14–24.

Diab, A., Ageez, A., & Gardoh, I. 2015. Serratia marcescens P25, A New Strain Isolated From The Phycoplane of the Red Marine Alga Punctaria sp Produced Potent Biosurfactant Used for Enhancing the Bioremediation of Spent Motor Oil-Polluted Soil.

Strobel, G., & Daisy, B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and molecular biology reviews, 67(4), 491-502.

Walsh, P. S., Metzger, D. A., & Higuchi, R. 2013. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 54(3), 134-139.

Kumar, S., Stecher, G., & Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution, 33(7), 1870-1874.

Saitou, N., & Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.

Drancourt, M., Berger, P., & Raoult, D. 2004. Systematic 16S rRNA gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans. Journal of clinical microbiology, 42(5), 2197-2202.

Offret, C., Desriac, F., Le Chevalier, P., Mounier, J., Jégou, C., & Fleury, Y. 2016. Spotlight on antimicrobial metabolites from the marine bacteria Pseudoalteromonas: chemodiversity and ecological significance. Marine drugs, 14(7), 129.

Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., and Yoshikawa, S. 1998. Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. International Journal of Systematic and Evolutionary Microbiology, 48(1), 247-256.

Bowman JP. Mar Drugs. 2007 Dec 18; 5(4):220-41

Lattasch, H.; Thomson, R.H. A revised structure for cycloprodigiosin. 1983. Tetrahedron Lett. 24, 2701–2704.

Holmström, C.; James, S.; Egan, S.; Kjelleberg, S. Inhibition of common fouling organisms by marine bacterial isolates ith special reference to the role of pigmented bacteria. Biofouling 1996, 10, 251–259.

Vynne, N.G.; Mansson, M.; Nielsen, K.F.; Gram, L. Bioactivity, chemical profiling, and 16S rRNA-based phylogeny of Pseudoalteromonas strains collected on a global research cruise. Mar. Biotechnol. 2011, 13, 1062–1073

Franks, A.; Haywood, P.; Holmström, C.; Egan, S.; Kjelleberg, S.; Kumar, N. Isolation and structure elucidation of a novel yellow pigment from the marine bacterium Pseudoalteromonas tunicata. Molecules 2005, 10, 1286–1291.



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