Activity of Moringa oleifera seed ethanolic extract against E. coli

Diah Ayu Nurafifah; Dyah Ayu Widyastuti; Ipah Budi Minarti

doi:10.26877/asset.v3i2.9603

Activity of Moringa oleifera seed ethanolic extract against E. coli

Diah Ayu Nurafifah, Dyah Ayu Widyastuti, Ipah Budi Minarti

Abstract

Bacterial contamination in our environment is worrying, mainly contamination at drinking waters, vegetables, foods, soil which close to our daily activity. The main contamination in environment is caused by E. coli which simply found easily surround us. So, it leads to bigger problem if not immediately solve. One of possible yet safe compounds to overcome this problem is the use of natural product such as M. oleifera seeds as antibacterial agents. This study want to find out the ability of M. oleifera seed ethanolic extract as antibacterial agent against E. coli. Ethanolic extract of M. oleifera seeds are concentrated into 25%, 50%, and 75%, then treated to E. coli culture under laboratory condition. The inhibitory zone diameter which formed after 24 hours incubation was measured and compared to control with no extract treatment. The result of this study showed that there is inhibitory zone formed in three groups of treatmen (T1-T3), but there is no inhibitory zone formed at control group. The 75% ethanolic extract of M. oleifera seeds (T3) has the wides inhibitory zone diameter among four groups, followed by the 50% extract (T2) and 25% extract (T1) with diameter 15,03 ± 0,55 mm; 11,00 ± 1,32 mm; 7,03 ± 0,90 mm, respectively. All inhibitory zone diameter among groups in this research statistically different with strong inhibitory status at T2 as well as T3, and moderate inhibitory status at T1.

Keywords

antibacterial; Escherichia coli; Moringa oleifera

Full Text:

PDF

References

Ercumen, A., Pickering, A. J., Kwong, L. H., Arnold, B. F., Parvez, S. M., Alam, M., Sen, D., Islam, S., Kullman, C., Chase, C., Ahmed, R., Unicomb, L., Luby, S. P., & Cloford, Jr., J. M. 2017. Animal feces contribute to domestic fecal contamination: Evidence from E. coli measured in water, hands, food, flies, and soil in Bangladesh. Environmental Science & Technology, 51, 8725-8734.

Aijuka, M., Santiago, A. E., Girón, J. A., Nataro, J. P., & Buys, E. M. 2018. Enteroaggregative Escherichia coli is the predominant diarrheagenic E. coli pathotype among irrigation water and food saources in South Africa. International Journal of Food Microbiology, 278, 44-51.

Leonard, A. F. C., Zhang, L., Balfour, A. J., Garside, R., Hawkey, P. M., Murray, A. K., Ukoumunne, O. C, & Gaze, W. H. 2018. Exposure to and colonisation by antibiotic-resistant E. coli in UK coastal water users: Environmental surveillance, exposure assessment, and epidemiological study (Beach Bum Survey). Environment International, 114, 326-333.

Ng, C., Chen, H., Goh, S. G., Haller, L., Wu, Z., Charles, F. R., Trottet, A., & Gin, K. 2018. Microbial water quality and the detection of multidrug resistant E. coli and antibiotic resistance genes in aquaculture sites of Singapore. Marine Pollution Bulletin, 135, 475-480.

Nowicki, S., deLaurent, Z. R., de Villiers, E. P., Githinji, G., & Charles, K. J. 2021. The utility of Escherichia coli as a contamination indicator for rural drinking water: Evidence from whole genome sequencing. PloS ONE, 16(1): e0245910. https:// doi.org/10.1371/journal.pone.0245910

Navab-Daneshmand, T., Friedrich, M. N. D., Gӓchter, M., Montealegre, M. C., Mlambo, L. S., Nhiwatiwa, T., Mosler, H., & Julian, T. R. 2018. Escherichia coli contamination across multiple environmental compartments (soil, hands, drinking water, and handwashing water) in Urban Harare: Correlations and risk factors. American Journal of Tropical Medicine and Hygiene, 98(3), 803-813. https://doi.org/10.4269/ajtmh.17-0521

Probert, W. S., Miller, G. M., & Ledin, K. E. 2017. Contaminated stream water as source for Escherichia coli O157 illness in children. Emerging Infectious Diseases, 23(7), 1216-1218.

Luna-Guevara, J. J., Arenas-Hernandez, M. M. P., Martinez de la Peña, Silva, J. L., & Luna-Guevara, M. L. 2019. The role of pathogenic E. coli in fresh vegetables: Behavior, contamination factors, and preventive measures. International Journal of Microbiology, 2019, 1-10. https://doi.org/10.1155/2019/2894328

Kholifah, Y. F., Dewi, E. R. S., & Widyastuti, D. A. 2019. Kemampuan daya hambat limbah kulit manggis (Garcinia mangostana L) sebagai antibakteri pada Bacillus cereus ATCC 10876. Prosiding Seminar Nasional Sains dan Entrepreneurship VI. ISBN 978-602-99975-3-8.

Nurdyansyah, F. & Widyastuti, D. A. 2020. Comparison of antioxidant activity of ethanolic, methanolic, n-hexan, and aqueous extract of Parkia speciosa peel based on half-maximal inhibitory concentration through free radical inhibition. Advance Sustainable Science, Engineering and Technology, 2(2), 0200207-01.

Nurdyansyah, F., Widyastuti, D. A., & Mandasari, A. A. 2021. Ethanolic extract effect of Parkia speciosa peel to hepar function used cooking oil exposed Rattus norvegicus. Jurnal Ilmu Kefarmasian Indonesia, 19(1), 111-117.

Katata-Seru, L., Moremedi, T, Aremu, O. S., & Bahadur, I. 2017. Green synthesis of iron nanoparticles using Moringa oleifera extracts and their applications: Removal of nitrate from water and antibacterial activity against Escherichia coli. Journal of Molecular Liquids, 2017. https://doi.org/10.1016/j.molliq.2017.11.093

Pritchard, M., Craven, T., Mkandawire, T., Edmondson, A. S., & O’Neill, J. G. 2010. A comparison between Moringa oleifera and chemical coagulants in the purification of drinking water – An alternative sustainable solution for developing countries. Physics and Chemistry of the Earth, 35, 798-805.

Walter, A., Samuel, W., Peter, A., & Joseph, O. 2011. Antibacterial activity of Moringa oleifera and Moringa stenopetala methanol and n-hexane seed extracts on bacteria implicated in water borne diseases. African Journal of Microbiology Research, 5(2), 153-157. https://doi.org/10.5897/AJMR10.457

Abalaka, M. E., Daniyan, S. Y., Oyeleke, S. B., & Adeyemo, S. O. 2012. The antibacterial evaluation of Moringa oleifera leaf extracts on selected bacterial pathogens. Journal of Microbiology Research, 2(2), 1-4.

Okuda, T., Baes, A. U., Nishijima, W., & Okada, M. 2001. Isolation and characterization of coagulant extracted from Moringa oleifera seed by salt solution. Water Research, 35(2), 405-410.

Widyastuti, D. A. & Rahayu, P. 2017. Antioxidant capacity comparison of ethanolic extract of soursop (Annona muricata Linn.) leaves and seeds as cancer prevention candidate. Biology, Medicine, & Natural Product Chemistry, 6(1), 1-4.

Gülçin, I., Küfrevioğlu, Ö, I., Oktay, M, Büyükokuroğlu, M. E. 2004. Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.) Journal of Ethnopharmacology, 90(2-3), 205-215. https://doi.org/10.1016/j.jep.2003.09.028

Ogbunugafor, H. A., Eneh, F. U., Ozumba, A. N., & Igwo-Ezikpe, M. N. 2011. Physico-chemical and antioxidant properties of Moringa oleifera seed oil. Pakistan Journal of Nutrition, 10(5), 409-414.

Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. 2007. Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research, 21, 17-25.

Akinyeye, A. J., Solanke, E. O., & Adebiyi, I. O. 2014. Phytochemical and antimicrobial evaluation of leaf and seed of Moringa oleifera extract. International Journal of Research in Medical and Health Sciences, 4(6), 1-10.

Okorondu, S. I., Akujobi, C. O., Okorondu, J. N., & Anyado-Nwadike, S. O. 2013. Antimicrobial activity of the leaf extracts of Moringa oleifera and Jatropha curcas on pathogenic bacteria. International Journal of Biological and Chemical Sciences, 7(1), 195-202.

Peixoto, J. R. O., Silva, G. C., Costa, R. A., de Sousa Fontenelle, J. L., Vieira, G. H. F., Filho, A. A. F., & dos Fernandes Vieira, R. H. S. In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extract. Asian Pasific Journal of Tropical Medicine, 4(3), 201-204. https://doi.org/10.1016/S1995-7645(11)60069-2

DOI: https://doi.org/10.26877/asset.v3i2.9603

Refbacks

There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Advance Sustainable Science, Engineering and Technology (ASSET)

E-ISSN: 2715-4211
Published by Science and Technology Research Centre

Universitas PGRI Semarang, Indonesia

Website: http://journal.upgris.ac.id/index.php/asset/index
Email: [email protected]