Liver Histopathology of Mice Induced by Paracetamol on Administration of Methanol Extract of Laban Tree Bark (Vitex pubescens Vahl.)

Wiwit Anggraini; Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo

doi:10.26877/bioma.v12i1.11664

Liver Histopathology of Mice Induced by Paracetamol on Administration of Methanol Extract of Laban Tree Bark (Vitex pubescens Vahl.)

Wiwit Anggraini, Diah Wulandari Rousdy, Elvi Rusmiyanto Pancaning Wardoyo

Abstract

Paracetamol metabolism takes place in the liver mediated by CYP450 enzymes and generates NAPQI radicals. The NAPQI radical is highly electrophilic to hepatocyte biomolecules and is therefore capable of inducing liver damage. Laban tree bark (V. pubescens) methanol extract is known to contain antioxidant compounds of flavonoids, saponins, and tannins with an IC₅₀ value of 19.83 g/ml. This study aims to determine the optimal dose of extract that can improve the liver microanatomy of mice induced by toxic dose of paracetamol (TDP). This study used 30 male Swiss strain mice with a body weight of 20-30 g. The treatments given consisted of negative control (paracetamol 105 mg kg^-1), positive control (vitamin E 46.8 mg kg^-1), and laban extract at a dose of 75; 150; 300; 600 mg kg^-1BW. Liver microanatomy parameters measured included hepatocyte diameter (19.50±0.21 µm), sinusoids (4.39±0.23 µm), and central vein (36.84 ± 1.79 µm). Data were analyzed using one-way ANOVA and Duncan's Multiple Range Test further test was then presented in the form of mean ± standard deviation. The results showed that extract at a dose of 600 mg kg^-1had hepatocyte, sinusoid, and central vein diameters that were not significantly different (p>0.05) with vitamin E.

Full Text:

PDF

References

Adelina, K., Wardenaar, E., & Sisillia, L. (2014). Kajian Etnobotani dan Fisiko Kimia Kulit Kayu Laban (Vitex Pubescens Vahl) di Desa Lape Kecamatan Kapuas Kabupaten Sanggau Kalimantan Barat. Jurnal Hutan Lestari, 2(1), 92. doi: http://dx.doi.org/10.26418/jhl.v2i1.5362

Ahmad, A. R., Elya, B., Mun’im, A. (2017). Antioxidant activity and Isolation of Xanthine Oxidase Inhibitor from Ruellia tuberosa Linn Leaf. Pharmacognosy Journal, 9(5), 607-610 doi: 10.5530/pj.2017.5.96

Al-Wajeeh, N. S., Halabi, M. F., Hajrezaie, M., Dhiyaaldeen, S. M., Bardi, D. A., Salam, S. M., Rouhollahi, E., Karimian, H., Abdolmalaki, R., Azizan, A. H., Ali, H. M., Noor, S. M., & Abdullah, M. A. (2016). The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damagein Sprague-Dawley Rats. Public Library of Science ONE, 11(9), 21. https://doi.org/10.1371/journal.pone.0157431

Bunchorntavakul, C., & Reddy, K. R. (2013). Acetaminophen - Related Hepatotoxicity. Clinic Liver Disease, 17(4), 587-607. DOI: 10.1016/j.cld.2013.07.005

Esrefuglo, M. (2013). Role of stem cells in repair of liver injury: Experimental and clinical benefit of transferred stem cells on liver failure. World Journal Gastroenterology, 19(40), 6757-6773. doi: 10.3748/wjg.v19.i40.6757

Garber, J. C., Barbee, R. W., Bielitzki, J. T., Clayton, L. A., Donovan, J. C., Kohn, F. F., Lipman, N. S., Locke, P., Melcher, J., Quimby, F. W., Turner, P. V., Wood, G. A., & Würbel, H. (2011). Guide for the Care and Use of Laboratory Animals. Washington DC: The National Academies Press.

Hermansyah, A., Harlia, & Zahara, T. A. (2015). Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Kulit Batang Laban (Vitex pubescens Vahl). Jurnal Kimia Khatulistiwa, 4(2), 67-71. https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/11735

Jaber, N. R., & Al-Bakri, N. A. (2018). Tegretol (Carbamazepine) Effect on the Morphometric Assay of Liver in Female White Mice (Mus musculus). Ibn Al-Haitham Journal for Pure & Applied Science, 31(3), 6. DOI: https://doi.org/10.30526/31.3.1997

Jun, M., Fu, H. Y., Hong, J., Wan, X., Yang, C. S., & Ho, T. (2003). Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria lobata Ohwi). Food Science Technologist, 68(6), 2117-2122. https://doi.org/10.1111/j.1365-2621.2003.tb07029.x

Jusup, I. (2014). Pengaruh Vitamin E dan Olahraga Terhadap Stres Oksidatif: Studi pada Mencit yang Terpapar Minyak Goreng Berulang. Nutrition and Health, 2(3). https://www.neliti.com/publications/89931/pengaruh-vitamin-e-dan-olahraga-terhadap-stres-oksidatif-studi-pada-mencit-yang

Katzung, B. G., Masters, S. B., & Trevor, A.J. (2014). Basic & Clinical Pharmacology, Edisi ke-12, New York: McGraw Hill Medical.

Makiyah, A., & Khumaisah, L. L. (2018). Studi Gambaran Histopatologi Hepar Tikus Putih Strain Wistar yang Diinduksi Aspirin Pascapemberian Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl.) Selama 7 Hari. Majalah Kedokteran Indonesia, 50(2), 93-101. doi: http://dx.doi.org/10.15395/mkb.v50n2.1323

Media, N., Rossiana, N., & Malini, D. M. (2020). Toksisitas Oil Sludge Hasil Fitoremediasi Long Term pada Hitologis Hati Mencit (Mus musculus). Jurnal Pendidikan dan Biologi, 12(2), 130-136. doi: https://doi.org/10.25134/quagga.v12i2.2621

Miller, M.A., Zachary, J.F. (2017). Mechanisms and Morphology of Cellular Injury, Adaptation, and Death. Pathologic Basis of Veterinary Disease 19: 2-43. doi: 10.1016/B978-0-323-35775-3.00001-1

Oertel, M., & Shafritz, D. A. (2008). Stem cells, cell transplantation and liver repopulation. Biochemistry Biophysiology Acta.February, 1782(2): 61–74. doi: 10.1016/j.bbadis.2007.12.004

Rousdy, D.W. & Wardoyo, E.R.P. (2018). Efek Antioksidan Fraksi Metanol Buah Lakum (Cayratia trifolia (L.) Domin) Terhadap Aktivitas Enzim Superoksida Dismutase dan Kadar Malondialdehid Pada Mencit Stres Fisik. Jurusan Biologi Fakultas MIPA Universitas Tanjungpura (Laporan Penelitian).

Safithri, F. (2018). Mekanisme Regenerasi Hati secara Endogen pada Fibrosis Hati. Malang: Fakultas Kedokteran Universitas Muhammadiyah.

Simanjutak, K. (2012). Peran Antioksidan Flavonoid dalam Meningkatkan Kesehatan. Jakarta: Bina Widya.

Suvarna, S.K., Layton, C., Bancroft, J.D. (ed.). (2013). Bancroft’s Theory and Practice of Histological Techniques. Churchill Livingstone Elsevier.

Treml, J., Smejkal, K., Hosek, J., & Zemlicka, M. (2013). Determination of Antioxidant Activity Using Oxidative Demage to Plasmid DNA-pursuit of solvent optimization. Chemical Papers, 67(5), 484-489. https://link.springer.com/article/10.2478/s11696-013-0334-8

Wilmana, P. F., & Gan, S. (2012). Analgesik-Antipiretik, Analgesik Antiinflamasi Nonsteroid, dan Obat Gangguan Sendi Lainnya. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

Wulandari, C., Rousdy, D.W., & Wardoyo, E.R.P. (2018). Skrining Fitokimia Berbagai Fraksi Ekstrak Buah Lakum (Cayratia trifolia L.). Jurnal Protobiont, 7(2), 66-70. doi: http://dx.doi.org/10.26418/protobiont.v7i2.26641

DOI: https://doi.org/10.26877/bioma.v12i1.11664