Optimasi dan uji efektivitas ekstrak Ganoderma lucidum sebagai anti-Helicobacter pylori

Intan Chairun Nisa, Brilliant Margalin


Helicobacter pylori diketahui sebagai penyebab utama tukak lambung dengan melemahkan lapisan pelindung pada lambung dan duodenum. Sejumlah obat anti tukak lambung yang sering digunakan dapat menyebabkan resistensi pada H. pylori. Ganoderma lucidum diketahui dapat menghambat dan mendukung penyembuhan tukak lambung yang disebabkan oleh asam asetat. Akan tetapi, kemampuan G. lucidum dalam menghambat tukak lambung yang disebabkan H. pylori belum banyak diungkap. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak G. lucidum dalam menghambat pertumbuhan H. pylori penyebab tukak lambung. Penelitian merupakan ekperimental dua faktorial yaitu jenis pelarut fraksinasi dan konsentrasi ektrak G. lucidum. Ekstrak G. lucidum difraksinasi menggunakan dua jenis pelarut yaitu etanol 60% dan akuades. Konsentrasi ekstrak G. lucidum yang digunakan adalah 1, 5, 10, 20, 30 mg/mL. Efektivitas ekstrak G. lucidum diuji dengan metode difusi cakram. Berdasarkan analisis statistik didapat bahwa jenis pelarut berpengaruh terhadap aktivitas daya hambat H. pylori dengan nilai pada pelarut etanol 60% signifikan lebih tinggi dibandingkan akuades. Konsentrasi ekstrak G. lucidum baik etanol maupun akuades berpengaruh signifikan terhadap aktivitas daya hambat. Aktivitas daya hambat tertinggi adalah pada perlakuan ekstrak etanol G. lucidum konsentrasi 20 mg/ml.


Kata kunci: akuades; difusi cakram; etanol; Helicobacter pylori; Ganoderma lucidum



Optimization and effectiveness assay of Ganoderma lucidum extract as Anti-Helicobactor pylori. Helicobacter pylori is known to be the main cause of gastric ulcers by weakening the protective lining of the stomach and duodenal. A number of gastric anti-ulcer drugs can cause resistance to H. pylori. Ganoderma lucidum is known to inhibit and support the healing of gastric ulcers caused by acetic acid. G. lucidum's ability to inhibit H. pylori growth has not been revealed much. This research aims to find out the effectiveness of G. lucidum extract in inhibiting the growth of H. pylori which causes gastric ulcers. This study is an experimental two factorial namely the type of fractionation solvent and the concentration of G. lucidum extract. Ganoderma lucidum extract is diffractionated using two types of solvents namely 60% ethanol and akuades. The concentration of G. lucidum extract used is 1, 5, 10, 20, 30 mg/mL. The effectiveness of G. lucidum is tested using the disc diffusion method. Based on statistical analysis found that the type of solvent affects the activity of H. pylori's resistance with a value in ethanol solvents 60% significantly higher than akuades On the other hand the concentration of G. lucidum extract in both ethanol and aquades has a significant effect on the activity of the slave. The highest inhibitory activity is in the treatment of ethanol extract G. lucidum concentration 20 mg / ml.


Keywords: aquades; diffusion disc; ethanol; Helicobacter pylori; Ganoderma lucidum

Full Text:



Bi, W. P., Man, H. B., & Man, M. Q. (2014). Efficacy and safety of herbal medicines in treating gastric ulcer: A review. World Journal of Gastroenterology, 20(45), 17020. http://doi.org/10.3748/wjg.v20.i45.17020

Feldman, M., Friedman, L. S., & Brandt, L. J. (Eds.). (2020). Sleisenger and Fordtran's gastrointestinal and liver disease. E-book: Pathophysiology, Piagnosis, Management. Elsevier.

Fernández-Agulló, A., Freire, M. S., & González-Álvarez, J. (2015). Effect of the extraction technique on the recovery of bioactive compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes. Industrial Crops and Products, 64, 105-113. https://doi.org/10.1016/j.indcrop.2014.11.031

Finch, R. G., Greenwood, D., Whitley, R. J., & Norrby, S. R. (2010). Antibiotic and chemotherapy e-book. Elsevier Health Sciences.

Gao Yihuai,Wenbo Tang, He Gao, Eli Chan, Jin Lan, and Shufeng Zhou. (2004). Ganoderma lucidum polysaccharide fractions accelerate healing of acetic acid-induced ulcers in rats. Journal of Medicinal Food, 7(4), 417–421. https://doi.org/10.1089/jmf.2004.7.417

Garrow, D., & Delegge, M. H. (2010). Risk factors for gastrointestinal ulcer disease in the US population. Digestive Diseases and Sciences, 55(1), 66. https://doi.org/10.1007/s10620-008-0708-x

Graham, D. Y. (2014). History of Helicobacter pylori, duodenal ulcer, gastric ulcer and gastric cancer. World Journal of Gastroenterology, 20(18), 5191. http://doi.org/ 10.3748/wjg.v20.i18.5191

Handrianto, P. (2017). Aktivitas antibakteri ekstrak jamur lingzhi (Gano lucidum) menggunakan pelarut etanol terhadap Escherichia coli. Journal of Pharmacy and Science, 2(1), 33-35. http://ejournal.akfarsurabaya.ac.id/index.php/jps/article/view/64

Hennicke, F., Cheikh-Ali, Z., Liebisch, T., Maciá-Vicente, J. G., Bode, H. B., & Piepenbring, M. (2016). Distinguishing commercially grown Ganoderma lucidum from Ganoderma lingzhi from Europe and East Asia on the basis of morphology, molecular phylogeny, and triterpenic acid profiles. Phytochemistry, 127, 29-37. http://doi.org/10.1016/j.phytochem.


Jorgensen, J. H., & Turnidge, J. D. (2015). Susceptibility test methods: dilution and disk diffusion methods. Manual of Clinical Microbiology, 71, 1253-1273. https://doi.org/10.1128/9781555817381.ch71

Koto, K., Asrul, A., & Muradi, A. (2016). Characteristic of gastric perforation type and the histopathology at Haji Adam Malik general hospital Medan-Indonesia. Bali Medical Journal, 5(1), 186-8. https://doi.org/ 10.15562/bmj.v5i1.325

Li, J., Xie, S., Ahmed, S., Wang, F., Gu, Y., Zhang, C., Chai, X., Wu, Y., Cai, J., & Cheng, G. (2017). Antimicrobial activity and resistance: Influencing factors. Frontiers in Pharmacology, 8, 364. . https://doi.org/10.3389/fphar.2017.00364

Liantari, D. S. (2014). Effect of wuluh starfruit leaf extract for Streptococcus mutans growth. Jurnal Majority, 3(7), 27-33. http://juke.kedokteran.unila.ac.id/index.php/majority/article/view/473

Martínez, L. E., Hardcastle, J. M., Wang, J., Pincus, Z., Tsang, J., Hoover, T. Bansil, R., & Salama, N. R. (2016). Helicobacter pylori strains vary cell shape and flagellum number to maintain robust motility in viscous environments. Molecular Microbiology, 99(1), 88-110. https://doi.org/10.1111/mmi.13218

Mégraud, F. (2004). H. pylori antibiotic resistance: Prevalence, importance, and advances in testing. Gut, 53(9), 1374-1384. http://dx.doi.org/10.1136/gut.2003.022111

Mobley, H. L., Mendz, G. L., & Hazell, S. L. (2001). Helicobacter pylori: Physiology and genetics. Retrieved from https://pubmed.ncbi.nlm.nih.gov/21290711/

Park, J. H., Jang, K. J., Kim, C-H., Lee, Y. H., Lee, S. J., Kim, B. H., & Yoon, H. M. (2014). Ganoderma lucidum pharmacopuncture for the treatment of acute gastric ulcers in rats. Journal of Pharmacopuncture, 17(3), 40–49. http://doi.org/10.3831/KPI.2014.17.025

Quereshi, S., Pandey, A. K., & Sandhu, S. S. (2010). Evaluation of antibacterial activity of different Ganoderma lucidum extracts. Journal of Scientific Research, 3(1), 9-13. https://www.researchgate.net/profile/Sardul-Sandhu/publication/267240166_Evaluation_of_antibacterial_activity_of_different_Ganoderma_lucidum_extracts/links/55ed94da08ae65b6389f5ea7/Evaluation-of-antibacterial-activity-of-different-Ganoderma-lucidum-extracts.pdf

Ruiz-Rico, M., Moreno, Y., & Barat, J. M. (2020). In vitro antimicrobial activity of immobilised essential oil components against Helicobacter pylori. World Journal of Microbiology and Biotechnology, 36(1), 1-9. http://doi.org/10.1007/s11274-019-2782-y

Shang, X., Tan, Q., Liu, R., Yu, K., Li, P., & Zhao, G. P. (2013). In vitro anti-Helicobacter pylori effects of medicinal mushroom extracts, with special emphasis on the Lion's Mane mushroom, Hericium erinaceus (higher Basidiomycetes). International Journal of Medicinal Mushrooms, 15(2), 165-174. http://doi.org/ 10.1615/IntJMedMushr.v15.i2.50

Sycuro, L. K., Pincus, Z., Gutierrez, K. D., Biboy, J., Stern, C. A., Vollmer, W., & Salama, N. R. (2010). Peptidoglycan crosslinking relaxation promotes Helicobacter pylori's helical shape and stomach colonization, Cell, 141(5), 822-833. http://doi.org/10.1016/j.cell.2010.03.046

Wiegand, I., Hilpert, K., & Hancock, R. E. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3(2), 163-175. https://doi.org/10.1038/nprot.2007.521

Yulianingtyas, A., & Kusmartono, B. (2016). Optimasi volume pelarut dan waktu maserasi pengambilan flavonoid daun belimbing wuluh (Averrhoa Bilimbi L.). Jurnal Teknik Kimia, 10(2), 61-67. https://media.neliti.com/media/publications/142082-ID-optimasi-volume-pelarut-dan-waktu-masera.pdf

DOI: https://doi.org/10.26877/bioma.v10i2.8236


  • There are currently no refbacks.

Journal has been indexed by:


Analytics View My Stats

Bioma : Jurnal Ilmiah Biologi by Biology Education  Universitas PGRI Semarang

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.