Aktivitas antibakteri metabolit sekunder isolat bakteri endofit kunyit (Curcuma longa L.) terhadap Propionibacterium acnes

Patrio Victorianus Baraga, Mahyarudin Mahyarudin, Ambar Rialita

Abstract


ABSTRAK

Propionibacterium acnes merupakan penyebab utama terjadinya acne vulgaris. Pengobatan tanda, gejala, dan kekambuhan acne vulgaris cukup sulit dikarenakan P. acnes yang resisten dengan antibakteri seperti golongan linkosamida. Kunyit (Curcuma longa L.) mempunyai senyawa aktif kurkumin yang merupakan polifenol alami dengan sifat antibakteri terhadap P. acnes. Bakteri endofit yang diisolasi dari suatu tanaman dapat menghasilkan metabolit sekunder yang sama dengan tanaman aslinya. Penelitian bertujuan untuk mengetahui aktivitas antibakteri metabolit sekunder endofit kunyit terhadap P. acnes. Penelitian bersifat deskriptif yaitu isolat bakteri endofit kunyit diujikan dengan metode difusi cakram terhadap P. acnes. Isolat yang paling berpotensi memiliki aktivitas antibakteri diidentifikasi berdasarkan karakterisasi morfologi koloni, morfologi sel, biokimia dan dilakukan uji senyawa metabolit sekunder dengan menggunakan metode Ciulei. Sebanyak 12 dari 17 isolat memiliki aktivitas terhadap P. acnes dengan diameter zona hambat terbedar yaitu 21,2 mm dengan kode isolat H5. Hasil identifikasi menunjukkan bahwa H5 memiliki kemiripan dengan genus Bacillus. Hasil uji metabolit menunjukkan bahwa senyawa antibakteri yang dihasilkan isolat H5 yaitu saponin, terpenoid dan flavanoid. Bakteri endofit tanaman kunyit memiliki potensi sebagai antibakteri terhadap bakteri P. acnes.

 

Kata kunci: antibakteri; bakteri endofit; Curcuma longa L.; Propionibacterium acnes.

 

 

ABSTRACT

Antibacterial activity of secondary metabolites of endophytic bacterial isolated from tumeric (Curcuma longa L.) against Propionibacterium acnes. Propionibacterium acnes is the main cause of acne vulgaris. Treatment to control the signs, symptoms, and recurrence of acne vulgaris is not easy due to its resistance to antibacterials such as linkosamide group. Turmeric (C. longa L.) has an active compound, curcumin, the main natural polyphenol which has antibacterial properties against P. acnes. Endophytic bacteria isolated from plant can produce same secondary metabolites with the host plant. The study aimed to determine the antibacterial activity of turmeric bacterial endophytes secondary metabolites against P. acnes. The research was descriptive research where endophytic bacterial isolates of turmeric were tested by disk diffusion method against P. acnes. The most potential isolates with antibacterial activity was identified based on the characterization of colony morphology, cell morphology, biochemical tests and a secondary metabolic compound test used Ciulei method. A total of 12 from 17 isolates had activity against P. acnes with the biggest inhibition zone is 21.2 mm with code H5. The identification results showed that H5 had similarities with the genus Bacillus. Metabolite test showed that the antibacterial compounds produced by H5 were saponins, terpenoids and flavonoids. The endophytic bacteria of turmeric plant has potential as an antibacterial against P. acnes.

 

Keywords: antibacterial; endophytic bacteria; Curcuma longa L.;  P. acnes.


Full Text:

PDF

References


Abdul, J. A., Posangi, J., Wowor, P. M., & Bara, R. A. (2020). Uji efek daya hambat jamur endofit rimpang jahe (Zingiber officinale Rosc) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Biomedik, 12(2), 88–93. https://doi.org/10.35790/jbm.12.2.2020.29163

Adityawarman. (2017). Isolasi, identifikasi dan aktivitas antibakteri endofit daun pegagang (Centella asiatica L.) terhadap Escherichia coli. Jurnal Cerebellum, 5(4B), 1569–1581. https://jurnal.untan.ac.id/index.php/JC/article/view/44821

Afzal, I., Shinwari, Z. K., Sikandar, S., & Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221, 36–49. https://doi.org/10.1016/j.micres.2019.02.001

Alshehri, M., Almutairi, A., Alomran, A., Alrashed, B., & Kaliyadan, F. (2017). Over-the-counter and prescription medications for acne: A cross-sectional survey in a sample of university students in Saudi Arabia. Indian Dermatology Online Journal, 8(2), 120. https://doi.org/10.4103/2229-5178.202273

Aydemir, E. H. (2017). Acne vulgaris. Türk Pediatri Arşivi, 49(1), 13–16. https://doi.org/10.5152/tpa.2014.1943

Becker, K., Skov, R. L., & von Eiff, C. (2015). Staphylococcus , Micrococcus , and other catalase-positive Cocci. In Manual of Clinical Microbiology (pp. 354–382). ASM Press. https://doi.org/10.1128/9781555817381.ch21

de Vero, L., Boniotti, M. B., Budroni, M., Buzzini, P., Cassanelli, S., Comunian, R., Gullo, M., Logrieco, A. F., Mannazzu, I., Musumeci, R., Perugini, I., Perrone, G., Pulvirenti, A., Romano, P., Turchetti, B., & Varese, G. C. (2019). Preservation, characterization and exploitation of microbial biodiversity: The Perspective of the Italian network of culture collections. Microorganisms, 7(12), 685. https://doi.org/10.3390/microorganisms7120685

Gouda, S., Das, G., Sen, S. K., Shin, H.-S., & Patra, J. K. (2016). Endophytes: A treasure house of bioactive compounds of medicinal importance. Frontiers in Microbiology, 7. https://doi.org/10.3389/fmicb.2016.01538

Guan, Z., Xue, D., Abdallah, I. I., Dijkshoorn, L., Setroikromo, R., Lv, G., & Quax, W. J. (2015). Metabolic engineering of Bacillus subtilis for terpenoid production. Applied Microbiology and Biotechnology, 99(22), 9395–9406. https://doi.org/10.1007/s00253-015-6950-1

Hewlings, S., & Kalman, D. (2017). Curcumin: A review of its effects on human health. Foods, 6(10), 92. https://doi.org/10.3390/foods6100092

Hidayat, S., Hanum, F., & Ismail, A. (2014). Efektivitas daya hambat dan daya bunuh bakteri ulkus traumatikus pada mukosa mulut dengan berbagai konsentrasi propolis (Trigona sp.). Medali: Media Dental Intelektual, 2(1), 79–84. http://jurnal.unissula.ac.id/index.php/medali/article/view/456/382

Horak, I., Engelbrecht, G., Rensburg, P. J. J., & Claassens, S. (2019). Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides. Journal of Applied Microbiology, 127(2), 326–343. https://doi.org/10.1111/jam.14218

Kaspar, F., Neubauer, P., & Gimpel, M. (2019). Bioactive secondary metabolites from Bacillus subtilis : A comprehensive review. Journal of Natural Products, 82(7), 2038–2053. https://doi.org/10.1021/acs.jnatprod.9b00110

Khan, M. I., Ahhmed, A., Shin, J. H., Baek, J. S., Kim, M. Y., & Kim, J. D. (2018). Green tea seed isolated saponins exerts antibacterial effects against various strains of Gram positive and Gram negative bacteria, a comprehensive study in vitro and in vivo. Evidence-Based Complementary and Alternative Medicine, 2018, 1–12. https://doi.org/10.1155/2018/3486106

Krieg, N. R., Staley, J. T., Brown, D. R., Hedlund, B. P., Paster, B. J., Ward, N. L., Ludwig, W., & Whitman, W. B. (2010). Bergey’s manual of systematic bacteriology second edition. In Springer-Verlag New York Inc.

Logan, N., & Vos, P. de. (2015). Bergeys’s manual of systematics of archaea and bacteria: genus bacillus (W. S. John, Ed.). Inc.

Mahmudah, F. L., & Atun, S. (2017). Uji aktivitas antibakteri dari ekstrak etanol temu kunci (Boesenbergia pandurata Roxb) terhadap bakteri Streptococcus mutans. Jurnal Penelitian Saintek, 22(1), 59. https://doi.org/10.21831/jps.v22i1.15380

Mamou, G., Malli Mohan, G. B., Rouvinski, A., Rosenberg, A., & Ben-Yehuda, S. (2016). Early developmental program shapes colony morphology in bacteria. Cell Reports, 14(8), 1850–1857. https://doi.org/10.1016/j.celrep.2016.01.071

Maulidia, V., Soesanto, L., Syamsuddin, S., Khairan, K., Hamaguchi, T., Hasegawa, K., & Sriwati, R. (2020). Secondary metabolites produced by endophytic bacteria against the root-knot nematode (Meloidogyne sp.). Biodiversitas Journal of Biological Diversity, 21(11). https://doi.org/10.13057/biodiv/d211130

Mollerup, S., Friis-Nielsen, J., Vinner, L., Hansen, T. A., Richter, S. R., Fridholm, H., Herrera, J. A. R., Lund, O., Brunak, S., Izarzugaza, J. M. G., Mourier, T., Nielsen, L. P., & Hansen, A. J. (2016). Propionibacterium acnes: Disease-causing agent or common contaminant? detection in diverse patient samples by next-generation sequencing. Journal of Clinical Microbiology, 54(4), 980–987. https://doi.org/10.1128/JCM.02723-15

Muharni, Fitrya, Oktaruliza, M., & Elfita. (2014). Uji aktivitas antibakteri dan antioksidan senyawa derivat piranon dari mikroba endofitik Penicillium sp. pada tumbuhan kunyit putih (Curcuma zedoaria (berg.) Roscoe). Traditional Medicine Journal, 19(3), 107–112. https://doi.org/10.22146/tradmedj.8226

Niederstebruch, N., Sixt, D., Benda, B. I., & Banboye, N. (2017). A suitable blood agar containing human blood especially for the use in laboratories of developing countries. The Journal of Infection in Developing Countries, 11(05), 399. https://doi.org/10.3855/jidc.8957

Nurhidayati, S., Faturrahman, F., & Ghazali, M. (2015). Deteksi bakteri patogen yang berasosiasi dengan Kappaphycus alvarezii (Doty) bergejala penyakit ice-ice. Jurnal Sains Teknologi & Lingkungan, 1(2), 24-30. https://doi.org/10.29303/jstl.v1i2.53

Nuria, M. C. (2010). Antibacterial activities from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves. Mediagro: Jurnal Ilmu-Ilmu Pertanian, 6(2), 9-15. https://doi.org/10.31942/md.v6i2.883

Nxumalo, C. I., Ngidi, L. S., Shandu, J. S. E., & Maliehe, T. S. (2020). Isolation of endophytic bacteria from the leaves of Anredera cordifolia CIX1 for metabolites and their biological activities. BMC Complementary Medicine and Therapies, 20(1), 300. https://doi.org/10.1186/s12906-020-03095-z

Palombo, E. A. (2020). Ethanol treatment does not inactivate spore-forming bacteria – A cautionary note about the safe transport of bacteria prior to identification by MALDI-TOF MS. Journal of Microbiological Methods, 172, 105893. https://doi.org/10.1016/j.mimet.2020.105893

Patel, J., Weinstein, M., Eliopoulus, G., Jenkins, S., Lewis, J., Limbago, B., & Mathers, A. (2017a). Performance Standards for Antimicrobial Susceptibility Testing (P. A. Wayne, Ed.; 27th ed., Vol. 37). Clinical and Laboratory Standards Institute.

Patel, J., Weinstein, M., Eliopoulus, G., Jenkins, S., Lewis, J., Limbago, B., & Mathers, A. (2017b). Performance Standards for Antimicrobial Susceptibility Testing (P. A. Wayne, Ed.; 27th ed., Vol. 37). Clinical and Laboratory Standards Institute.

Pence, M. A., & Liesman, R. (2020). Clinical microbiology. In Contemporary Practice in Clinical Chemistry (pp. 985–1006). Elsevier. https://doi.org/10.1016/B978-0-12-815499-1.00055-7

Rahmadani, B. A. (2018). Karakteristik bakteri endofit daun pare (Momordica charantia L.) yang memiliki aktivitas antibakteri terhadap Shigella flexneri. Jurnal Mahasiswa PSPD FK Universitas Tanjungpura, 4(1), 3–22. https://jurnal.untan.ac.id/index.php/jfk/article/view/25678

Septiana, E., Sukarno, N., & Simanjuntak, P. (2017). Endophytic fungi associated with turmeric (Curcuma longa L.) can inhibit histamine-forming bacteria in fish. . HAYATI: Journal of Biosciences, 24(1), 46–47. https://doi.org/10.4308/hjb.24.1.46

Sharma, D., Pramanik, A., & Agrawal, P. K. (2016). Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D.Don. 3 Biotech, 6(2), 210. https://doi.org/10.1007/s13205-016-0518-3

Soleimani, V., Sahebkar, A., & Hosseinzadeh, H. (2018). Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances: Review. Phytotherapy Research, 32(6), 985–995. https://doi.org/10.1002/ptr.6054

Sulistiyani, S., Ardyati, T., & Winarsih, S. (2016). Antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. The Journal of Experimental Life Sciences, 6(1), 45–51. https://doi.org/10.21776/ub.jels.2016.006.01.11

Yuanita, M. M., Putri, E. A., Mahyarudin, M., Mardhia, M., & Rialita, A. (2019). Eksplorasi bakteri gram negatif endofit tanaman kunyit (Curcuma longa L.) yang memiliki kemampuan quorum quenching. Majalah Kedokteran Andalas, 42(2), 80. https://doi.org/10.25077/mka.v42.i2.p80-90.2019

Yusmaniar, Wardiyah, & Nida, K. (2017). Mikrobiologi dan parasitologi (E. Krisnadi, Ed.; 1st ed.). Kementrian Kesehatan Republik Indonesia. http://bppsdmk.kemkes.go.id/pusdiksdmk/wp-content/uploads/2017/11/DAFTAR-ISI-DAN-MIKROBIOLOGI-PARASITOLOGI.pdf

Zhu, T., Zhu, W., Wang, Q., He, L., Wu, W., Liu, J., Li, Y., & Sun, D. (2019). Antibiotic susceptibility of Propionibacterium acnes isolated from patients with acne in a public hospital in Southwest China: Prospective cross-sectional study. BMJ Open, 9(2), e022938. https://doi.org/10.1136/bmjopen-2018-022938




DOI: https://doi.org/10.26877/bioma.v11i1.10558

Refbacks

  • There are currently no refbacks.


Journal has been indexed by:

 


Web
Analytics View My Stats



Bioma : Jurnal Ilmiah Biologi by Biology Education  Universitas PGRI Semarang

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.